Tissue-specific intergenic transcripts throughout the human apoAI
cluster. A, schematic map of the apoAI locus indicating the sites
assayed. The primer pairs of 3P represent the site at the apoCIII promoter.
The primer pairs of I54, I43, and I31 represent the sites in
the intergenic region of apoAV-AIV, apoAIV-CIII, and apoCIII-AI, respectively.
The primer pairs of U5 represent the site in the intergenic region
between apoAV and ZNF259. B, intergenic transcripts of the apoAI gene
cluster were expressed specifically in HepG2 and Caco-2 cells. C,
intergenic transcripts of the apoAI cluster were expressed specifically in the
liver and the intestine of transgenic mice. + and - represent the reverse
transcription with or without avian myeloblastosis virus reverse
transcriptase. The ladder for DL2000 marker is 100, 250, 500, 750, 1000, and
2000 bp, respectively (low to high).