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. 2008 Oct 17;283(42):28595–28606. doi: 10.1074/jbc.M805766200

FIGURE 7.

FIGURE 7.

Effect of oxypurinol pre-treatment on RV16-induced chemokine (IL-8 and GRO-α) production and on NF-κB transcription factor activation. A (n = 5) and B (n = 5), A549 cells were exposed for 1 h to medium alone (Medium), RV16 physically removed by filtration (f-RV16) or live RV16 (RV16). Where indicated cells were pretreated for 4 h with oxypurinol (Oxy+RV16) or diluent (Diluent+RV16) before the infection. Supernatants were harvested at 4 h and levels of IL-8 (panel A) and GRO-α (panel B) were assessed (***, p < 0.001). C, A549 cells were exposed for 20 min to RV16 with (dark gray bar) or without (black bar) 20 μm oxypurinol (Oxy) after a 4-h pretreatment and NF-κB activation was assessed in nuclear extracts (n = 3, *, p < 0.05 versus medium alone).