Effect of oxypurinol pre-treatment on RV16-induced chemokine (IL-8 and
GRO-α) production and on NF-κB transcription factor
activation. A (n = 5) and B (n = 5),
A549 cells were exposed for 1 h to medium alone (Medium), RV16
physically removed by filtration (f-RV16) or live RV16
(RV16). Where indicated cells were pretreated for 4 h with oxypurinol
(Oxy+RV16) or diluent (Diluent+RV16)
before the infection. Supernatants were harvested at 4 h and levels of IL-8
(panel A) and GRO-α (panel B) were assessed
(***, p < 0.001). C, A549 cells were exposed
for 20 min to RV16 with (dark gray bar) or without (black
bar) 20 μm oxypurinol (Oxy) after a 4-h
pretreatment and NF-κB activation was assessed in nuclear extracts
(n = 3, *, p < 0.05 versus medium
alone).