TABLE 1.

Kinetic constants for binding of choline to LytC measured by spectropolarimetry

Measurements were at 4 °C in P_i buffer plus 140 mm choline.

λ (nm)	Fast phase		Slow phase
	k1	ΔΘ1a	k2	ΔΘ2a
	min−1	%	min−1	%
225	0.3 ± 0.1	34 ± 4	0.023 ± 0.003	66 ± 7
285	0.4 ± 0.1	26 ± 3	0.015 ± 0.002	74 ± 7

^aΔΘ₁ and ΔΘ₂ are the relative ellipticity changes associated with the fast and the slow phase, respectively.