TABLE 4.

Thermodynamic parameters associated with choline binding to Cpl-1

Measurements were in P_i buffer at 25 °C. Dimerization parameters are expressed per mole of dimer, and the binding parameters are expressed per mole of binding site.

	ΔGa	ΔH	ΔSb
	kcal/mol	kcal/mol	cal/(mol.K)
Dimerization	−3.9 ± 0.2	−75 ± 10	−240 ± 30
Binding	−3.7 ± 0.2	4 ± 1	26 ± 4

^aΔG = - RTlnK (K = or K_B); R is the gas constant, T is the temperature in Kelvin, and and K_B are, respectively, the dimerization constant and the intrinsic affinity constant for choline binding (see Scheme 1).

^bΔS = (ΔH - ΔG)/T.