Human tissue eosinophils from infected CF patients contain exclusively
3NT-positive residues. A and B, fluorescence micrograph
of 3NT-positive cells in the lung mucosa (panel B, M) and in the
sputum (panel B, S) filled bronchial lumen (panel B, L) of a
patient with CF stained with a rabbit NT antibody (A) and DAPI
(B)(panel B, E (epithelium)), original magnification:
A and B, ×100. Double fluorescence micrographs of CF
tissue sections stained with a rabbit antibody against 3NT (panels C, E,
G, I, and K) and markers specific for eosinophils (D),
T-lymphocytes (F), mast cells (H), monocytes (J),
and neutrophils (L). Only eosinophils were 3NT-positive (panels
C and D, arrows). Original magnification: panels C-L,
×400.