FIGURE 2.

Human blood eosinophils from inflamed and healthy individuals are exclusively 3NT-positive. Double fluorescence micrographs of granulocyte and lymphocyte preparations of a patient with atopic rhinitis (A) and a healthy individual (B) were stained with a rabbit antibody against 3NT (green) and cell-specific monoclonal antibodies against MBP (red) for eosinophils, neutrophil elastase for neutrophils, and CD3 for T-lymphocytes. Additionally, cells were stained with DAPI. The overlay shows perfect co-localization of MBP and 3NT in eosinophils but no co-localization of neutrohil elastase with 3NT or presence of 3NT in lymphocytes. C, in lung tissue of a healthy individual NT-positive cells are present in the lung mucosa (M)(L, lumen; E, epithelium). Original magnification: C, ×100. D, the overlay of double-stained blood smears of a patient with atopic rhinitis and a healthy individual show double staining exclusively for eosinophil cells. Original magnification: A, B, and D, ×400.