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. 2008 Oct 17;283(42):28629–28640. doi: 10.1074/jbc.M801196200

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Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Tyrosine nitration of eosinophil granule toxins from human umbilical cord stem cells, IL-5/gp91^phox^-^/^- mice, and mice lacking nitric-oxide synthases. A, cord blood eosinophils after in vitro culture at days 1, 3, 7, and 14 stained for 3NT (green). Original magnification: ×1000. 3NT-negative cord blood cells became 3NT-positive at day 3. B, rapid tyrosine nitration in vitro is dependent on hydrogen peroxide and nitrite. Dot blot shows autonitration of IL-5/gp91^phox^-/- mouse granula toxins at 5, 10, 15, and 30 s. C, blood smears from mice lacking iNOS (left), nNOS and eNOS (middle), and iNOS/eNOS/nNOS (right), stained with antibodies specific for MBP (red), 3NT (green), and with DAPI. Original magnification: ×1000.