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. Author manuscript; available in PMC: 2010 Feb 15.
Published in final edited form as: Free Radic Biol Med. 2008 Nov 7;46(4):454–461. doi: 10.1016/j.freeradbiomed.2008.10.046

Fig. 1. Immunological detection of DMPO-protein adducts in spleen tissue homogenates.

Fig. 1

Fig. 1

Spleen tissue homogenates from mice treated as described in the materials and methods were examined by immunological methods and Coomassie blue staining. A) ELISA analysis using anti-DMPO polyclonal rabbit antiserum showing values significantly different (P<0.05) from the sham sample indicated by an asterisk (*) and values significantly different (P<0.05) from the sham+DMPO sample indicated by a #. B) Representative Western blots analyzed with anti-actin antibody (top panel) as a loading control and anti-DMPO antibody (middle-panel). The bottom panel shows the relative band intensity of the data from the anti-DMPO Western blot. Values represent the mean ± SE, with values significantly different (P<0.05) from the sham sample indicated by an asterisk (*) and values significantly different (P<0.05) from the sham+ DMPO sample indicated by #. C) Coomassie blue-stained gel indicating a 47 kD band used for MALDI-TOF.