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. Author manuscript; available in PMC: 2010 Feb 15.
Published in final edited form as: Free Radic Biol Med. 2008 Nov 7;46(4):454–461. doi: 10.1016/j.freeradbiomed.2008.10.046

Fig. 2. Immunoprecipitation of DMPO-adducts.

Fig. 2

Proteins were immunoprecipitated from spleen tissue homogenates using either normal mouse IgG as a control or with anti-DMPO mouse IgG or goat anti-CPB1. A) Western blot analysis of immunoprecipitates using goat anti-CPB1. Lane 1, normal mouse IgG; lane 2, anti-DMPO mouse IgG; lane 3, goat anti-CPB1. B) Western analysis of immunoprecipitates using rabbit polyclonal anti-DMPO. Lane 1, normal mouse IgG; lane 2, anti-DMPO mouse IgG. Blots are representative of 3 different immunoblots from an equal number of experiments.