Fig. 3. Effect of allopurinol inhibition of xanthine oxidase on CPB1-DMPO adduct formation.
Protein from spleen tissue homogenates from mice treated as shown were immunoprecipitated using anti-DMPO and were analyzed by Western blotting using anti-CPB1 (top panel). The Western blot represents results from 3 independent experiments with the best figure being displayed. Relative band intensities from the scanned Western blot are shown in the bottom panel. Values significantly different (P<0.05) from the sham+DMPO sample are indicated by an asterisk (*) while values significantly different (P<0.05) from the LPS+DMPO sample are indicated by #.