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. Author manuscript; available in PMC: 2010 Jan 23.
Published in final edited form as: Neuroscience. 2008 Nov 7;158(2):922–931. doi: 10.1016/j.neuroscience.2008.10.050

Figure 6.

Figure 6

Substance P and NK-1 immunostaining in the superficial lamina of dorsal horns in cervical spinal cord segments. (A) Dorsal horns of week 6 MRHF rats have punctuate substance P immunofluorescence (green) staining that is distributed across the entire zone, medial to lateral, of the superficial lamina with increased expression more laterally. Medial (med) and lateral (lat) regions of the superficial lamina are indicated. Inset in panel (A) shows a C7 spinal cord cross-section at low power. (B) Dorsal horns of control rats have low levels of substance P immunofluorescence staining in the superficial lamina. (C) Dorsal horns of week 6 MRHF rats have NK-1 immunofluorescence (red) staining on plasma membranes and dendrites with some endosome swellings that spans the entire zone of the superficial lamina in which increased expression is observed more medially. (D) Dorsal horns of control rats have low levels of NK-1 immunofluorescence staining in the dorsal horn superficial lamina. Bar = 10 μm. D and E) Mean (+ SEM) percent immunofluorescent staining for substance P and neurokinin1 in the spinal cord dorsal horn superficial lamina. Normal and trained controls (C, n=4 each group for a total of 8 control rats) and rats that had performed the MRHF task for 6 weeks (n=4) or 12 weeks (n=4) were quantified. Significant increases from control levels are denoted by symbols (*p<0.01 and **p<0.001 compared to controls).