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TCF7L2 expression does not affect the morphologically docked granule pool. A: Mouse islets were transfected with siRNA as described. The total number of granules, morphologically docked, and within 200 nm of the membrane pools were analyzed as described in research design and methods; n = 14–16 cells per condition. B and C: Representative β-cells with examples of docked granules and mitochondria (scrambled siRNA [B] and TCF7L2 siRNA [C]). Scale bars = 2 μm.
