FIG. 5.

Castration reverses gene expression patterns in male and female adipocytes. Male and female mice were castrated (C) or sham operated (S). Six weeks after surgery, adipocytes from PG and SC fat were isolated, mRNA was extracted, and quantitative real-time PCR was performed (A). Gene expression for metabolic genes was assessed and normalized against the expression of TATA-binding protein. B: Proteins from similar cells were subjected to SDS-PAGE and Western blot with the respective antibodies. Actin was used as a control for protein loading. C: Quantitation of the proteins was achieved by densitometry normalized by the values for actin. *P < 0.05. IR, insulin receptor.