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. 2009 Jan 9;58(4):855–866. doi: 10.2337/db08-1644

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© 2009 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 1. — Desnutrin overexpression in adipose tissue of mice. A: Transgene expression was verified by RT-PCR in tissues from aP2-desnutrin mice. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. B: Desnutrin mRNA level in gonadal WAT and BAT as determined by RT-qPCR (n = 5–6). □, wild type; ■, aP2-desnutrin. C: CGI-58 mRNA level in gonadal WAT as determined by RT-qPCR (n = 6–7). D: Immunoblot and quantification of desnutrin-HA fusion protein in gonadal WAT and BAT. □, endogenous; ■, endogenous + transgene. E: TAG lipase activity in WAT homogenates from wild-type and aP2-desnutrin mice. *P < 0.05, **P < 0.01, ***P < 0.001. All data are from female mice. WT, wild type. (A high-quality digital representation of this figure is available in the online issue.)