Figure 2.

The level of isopentenylated tRNA found in ALB1 overexpressing ERG20 is reduced substantially. (A) Low-molecular-weight RNA was prepared from ALB1 (mod5-M2) with each of the candidate genes or vector alone, ALB8 (MOD5), or MD14A (mod5–1). The RNAs were resolved on polyacrylamide gels, transferred to membranes, and probed with anti-isopentenyl adenosine antibody (Upper) or radiolabeled oligonucleotide complementary to mature tRNA^Tyr (Lower). (B) The levels of isopentenyl adenosine tRNA found in ALB1 with each of the candidate genes or vector only or in the strain ALB8 or MD14A were assessed by densitometric analysis of two immunoblots and expressed as a fraction of the level found in the “vector” control. A, membrane 1 values; B, membrane 2 values; C, average values.