Skip to main content
. 2009 Mar 15;23(6):729–739. doi: 10.1101/gad.1770309

Figure 4.

Figure 4.

(A) NBA1 is required for proper G2–M regulation in response to IR. U2OS cells were untreated or treated with 3 Gy IR, then incubated for 1 h at 37°C before fixation and p-H3 antibody staining. Two independent experiments were performed with two different pairs of siRNAs to NBA1 and BRCC36. (B) NBA1 is required for optitmal BRCA1 and Rap80 foci formation in response to IR. U2OS cells were transfected with control or siRNAs against NBA1, BRCC36, or BRE for 2 d, then irradiated with 10 Gy IR, incubated for 2 h, fixed and immunostained with the indicated antibodies. (C) The defect in BRCA1 IRIF formation in cells expressing shRNA to NBA1 can be rescued by expression of an shRNA-resistant NBA1 cDNA. U2OS cells expressing NBA1 shRNA#1 were infected with virus expressing NBA1 cDNA lacking the target sequence of shRNA#1, irradiated with 10 Gy IR, incubated for 4 h, fixed, and stained with Brca1 antibodies.