Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Mar 15;23(6):729–739. doi: 10.1101/gad.1770309

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2009 by Cold Spring Harbor Laboratory Press

Freely available online through the Genes & Development Open Access option.

PMC Copyright notice

Figure 5. — NBA1 serves as an adaptor protein for the BRCA1 A complex. (A) NBA1 and BRE proteins interact with the N terminus of Abra1. Cell lysates from 293T cells stably expressing GFP-tagged mutants of Abra1 were immunoprecipitated with GFP antibodies and immunoblot analysis was carried out with antibodies against NBA1, BRE, or BRCC36. (B) A diagram for Abra1 deletion mutants. (C) NBA1–Rap80 interaction, similar to BRCC36–Rap80 interaction, depends on the AIR domain of Rap80 that is required for Rap80–Abra1 interaction. Cell lysates from 293T cells stably expressing GFP-tagged mutants of Rap80 were immunoprecipitated with GFP antibodies and Western blot was carried out with antibodies against NBA1 or BRCC36. (D) A diagram for Rap80 deletion mutants. (E) NBA1 and BRE interaction with BRCC36 is mediated through Abra1. Cell lysates from 293T cells stably expressing GFP-tagged NBA1 or BRE protein were immunoprecipitated with GFP antibodies and Western blot was carried out with antibodies against Abra1, BRE, or BRCC36. (F) A diagram mapping the regions of Abra1 that interacts with different members of the BRCA1-A complex.