Figure 6.

Members of the BRCA1 A complex contain ubiquitin-binding domains. (A) Alignment of the VWA domain of NBA1 and PSMD4/Rpn10. (B) Alignment of the MPN domains from various species of Abra1, Abro1, and other MPN domain-containing proteins. Residues outlined in red are required for enzymatic activity. (C) Alignment of the UEV domain from BRE with the UEV domain of human E2-conjugating enzyme Rad6a and UbcH5. (D) Protein domains present in the components of BRCA1 A complex. (UIM) Ubiquitin-interacting motif; (AIR) Abraxas-interacting region; (MPN−) Mpr1/Pad1 N-terminal region lacking catalytic activity; (MPN+) Mpr1/Pad1 N-terminal region with an intact protease catalytic site; (CC) coiled coil; (UEV) ubiquitin-conjugating enzyme variant domain; (VWA) von Willebrand factor type A domain.