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. 2009 Mar 15;23(6):681–693. doi: 10.1101/gad.1773109

Figure 5.

Figure 5.

cJun and TEL sites are sufficient to confer NCoR and SMRT binding to the Il12b and Mmp13 promoters, respectively. (A) Schematic diagram of the wild-type and mutant Mmp13 and Il12b promoters indicating the swapped AP1 and ETS sites. ETS-T is the TEL-binding site in the Il12b promoter. ETS-A is a binding site for ETS activators in the Mmp13 promoter. AP-1 is the cJun-binding site in the Mmp13 promoter. (B,C) Replacing the ETS-A site in the Mmp13 promoter with the ETS-T site from Il12b inhibits the recruitment of NCoR and promotes SMRT binding to the Mmp13 promoter as shown by ChIP assays performed in RAW cells transfected with the plasmids shown in A. (D,E) NCoR is recruited to the Il12b promoter upon a swap of the AP1 Mmp13 site into the ETS-T on the ETS site as shown by ChIP assays. (*) P < 0.05 versus wild type. Results are expressed as the average of three independent experiments. Error bars represent standard deviations. (F,G) Functional analysis of the indicated wild-type and mutant Mmp13 and Il12b promoters in RAW264.7 cells.