(a) A central section through the virion–sPvr reconstruction, normal to a 2-fold symmetry axis. The boxed region is shown in b. High density is shown as dark. The right edge of the box coincides with a 5-fold symmetry axis. (b) The virion–sPvr (Top) and virion (Bottom, from ref. 9) maps were oriented as in a rotated progressively about the 5-fold symmetry axis. (Left to Right) The rotation is −18°, 0°, 6°, 12°, 24°, and 30°. In each case, the boxed portion of the central section was extracted. Arrows indicate sPvr-related density that bridges the canyon (second panel from right) and the solvent-level density corresponding to the tunnel (rightmost panel). Bars = 50 Å.