FIGURE 1.

Knockdown of drosha in S2 cells. (A) Schematics of the utilized strategy. Pri-miRNAs are transcribed from intergenic regions (miRNA genes) or contained in introns of protein coding genes (PCG). These pri-miRNAs are cleaved by a complex containing drosha and pasha. Inhibition of this pathway by the use of drosha dsRNA should result in pri-miRNA accumulation. (B) Incubation with drosha dsRNA reduces drosha mRNA levels. The graph represents the difference of expression between S2 cells treated with control (luciferase) or drosha dsRNA in the genomic region that contains drosha. Two samples for each condition were performed. The dotted red circles indicate the region of drosha to which the dsRNAs were directed. (C) Quantitative RT-PCR using primers for drosha shows that the level of drosha RNA decreases significantly in S2 cells upon treatment with two different combinations of dsRNAs against drosha.