FIGURE 6.

MimiTgs is a functional ortholog of S. cerevisiae Tgs1. (A) Yeast tgs1Δ mud2Δ p (CEN URA3 TGS1) cells were transformed with a CEN LEU2 plasmid bearing wild-type S. cerevisiae TGS1 (positive control), an empty 2μ LEU2 vector (negative control), and 2μ LEU2 plasmids expressing either wild-type MimiTGS or MimiTGS-D179A. Leu⁺ transformants were selected at 30°C and then streaked to agar medium containing FOA. The plate was photographed after 3 d at 30°C. (B) Yeast tgs1Δ cells were transformed with a CEN LEU2 plasmid bearing wild-type S. cerevisiae TGS1 (positive control), an empty 2μ LEU2 vector (negative control), and a 2μ LEU2 plasmid expressing MimiTGS. Leu⁺ transformants were selected at 30°C and then tested for growth at 30°C and 18°C by spotting serial 10-fold dilutions of liquid cultures (grown at 30°C in SD-Leu medium) on Leu^– agar plates. The plates were photographed after incubation for 3 d at 30°C or 7 d at 18°C.