FIGURE 2.

siLam delivered by PS-ON colocalizes with the smooth ER marker AMF but not with the Golgi marker CTxB. ECV-304 cells were simultaneously treated with 1 μM PS-ON 72mer, 1 μM siLam-Alexa488 (green) or -Alexa555 (red), and 10 μg/mL AMF-Cy3 (red; A) or 0.5 μg/mL CTxB-FITC (green; B), respectively, for 60 min. Afterward, cells were extensively washed with Opti-MEM and nuclei (blue) were stained with Hoechst 33342. Optical sections were acquired 0, 2, 6, or 24 h after the treatment. Bars, 10 μm.