Fig. 5.
Ik2 and Kat-60L1 are not required for the axon and dendrite pruning in MB γ neurons. (A) Schematic diagram of MB γ neurons at larval, pupal and adult stages. The MB γ neurons contains dendrites (arrowheads) and dorsal (d) and medial (m) axonal branches (solid arrows) in larvae. At 18 h APF, both axonal branches were pruned (dashed arrows). Only the medial axon (solid arrow) re-extend to the midline in adults. At 18 h APF, both axon branches (dashed arrows) and dendrites (arrowhead) of γ neurons pruned in the wild-type MARCM clones (B), and in the ik2 MARCM clones (C). Nearby non-γ neurons were indicated by asterisks. 2-cell wild type (D) and ik2 (E) MARCM clones of γ neuron regrew their dendrites (arrowhead) and medial axons (solid arrow) in adult. MB γ neurons marked by201Y-GAL4 and UAS-mCD8GFP at 18 h APF (F and G) and also by anti-Fas II antibody staining in adults (H and I), showed normal axon pruning in kat-60L1c01236 mutants (G and I), compared to wild type (F and H). n ≥ 8 clones (B–E), and n ≥ 10 brains (F–I). (Scale bar, 50 μm.)