Skip to main content
. Author manuscript; available in PMC: 2009 Mar 27.
Published in final edited form as: Neuron. 2007 Jul 5;55(1):53–68. doi: 10.1016/j.neuron.2007.05.030

Figure 3. BDNF-dependent Formation and Polarization of TrkB Signaling Endosome in vivo.

Figure 3

Cerebellar sections from P6 bdnf +/+ or −/− mice were used for immunostaining as indicated (A and B). DAPI staining visualizes nuclei (blue). Scale bars: 5 μm.

(A) BDNF-dependent polarization of TrkB receptors in vivo. TrkB receptors at the front of GCPs in EGLb are seen within a crescent or extended leading process in bdnf +/+ mice (A1–A4), but are more randomly distributed within the cells of bdnf −/− mice (A5–A8), as visualized by immunostaining with anti-TrkB (red) and anti-zic (green). A4 and A8 are magnified view of boxed region in A3 and A7, respectively.

(B) BDNF-dependent formation and polarization of TrkB signaling endosomes in vivo. TrkB receptors colocalize with α-adaptin at leading edge of GCPs from bdnf +/+ mice (B1–B4), but are distributed in random patterns in bdnf −/− GCPs (B5–B8). Colocalization and polarization of TrkB and α-adaptin was visualized by immunostaining with anti-TrkB (red) and anti-α-adaptin (green), respectively. B4 and B8 are magnified view of boxed region of B3 and B7, respectively.

(C) Schematic of TrkB immunostaining pattern in the inner EGL (EGLb) of sagittal sections of developing cerebellum. Cells marked “+” would be scored as correctly polarized. TrkB (red) and Zic (blue).

(D) Quantitative analysis of TrkB polarization toward IGL in EGLb in bdnf +/+ and −/− mice. Data shown represent the means ± SEM and were evaluated by two-tailed Student’s t-test (*, p < 0.001; n=6).