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Tyrosine phosphopeptides are identified in ALK+ ALCL cells. (A) MS/MS spectrum of tyrosine-phosphorylated peptide VVACNLYPFVK, assigned to the protein ATIC (percentage coverage, 1.8% of total sequence). (B) MS/MS spectrum of tyrosine-phosphorylated peptide VQIYHNPTANSFR, assigned to the protein VASP (percentage coverage, 3.4% of total sequence). (C) Total protein expressions (top panel) and phosphorylation status (bottom panel) as assayed by Western blot with the indicated antibodies.
