Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1993 Nov;31(11):3001–3006. doi: 10.1128/jcm.31.11.3001-3006.1993

Typing of strains from a single-source outbreak of Pseudomonas pickettii.

W J Dimech 1, A G Hellyar 1, M Kotiw 1, D Marcon 1, S Ellis 1, M Carson 1
PMCID: PMC266187  PMID: 8263186

Abstract

Plasmid profiles, genome restriction fragment polymorphisms, carbohydrate oxidation-fermentation reactions, methylumbelliferyl substrate hydrolysis patterns, antimicrobial susceptibilities, and results obtained with the Biolog GN biochemical substrate kit were used to type 19 common-source, but mixed-biotype, outbreak strains and one epidemiologically distinct strain of Pseudomonas pickettii. Biotyping with conventional and methylumbelliferyl substrates failed to distinguish between strains. Plasmid profile testing was found to be inconsistent and not reproducible. The Biolog GN kit allowed greater strain differentiation than restriction fragment polymorphism did (12 biotypes versus 5 biotypes); antimicrobial susceptibility testing yielded 4 biotypes, and oxidation-fermentation tests gave 3 biotypes. Oxidation-fermentation results were consistent with restriction fragment polymorphs in all but 1 of the 20 strains tested. For ease of typing, comprehensive typeability, and reproducibility, oxidation-fermentation tests should be performed initially and followed if necessary by restriction fragment polymorph analysis for the elucidation of P. pickettii infection outbreaks.

Full text

PDF
3001

Images in this article

3004Image
on p.3004

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson D. J., Kuhns J. S., Vasil M. L., Gerding D. N., Janoff E. N. DNA fingerprinting by pulsed field gel electrophoresis and ribotyping to distinguish Pseudomonas cepacia isolates from a nosocomial outbreak. J Clin Microbiol. 1991 Mar;29(3):648–649. doi: 10.1128/jcm.29.3.648-649.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson R. L., Holland B. W., Carr J. K., Bond W. W., Favero M. S. Effect of disinfectants on pseudomonads colonized on the interior surface of PVC pipes. Am J Public Health. 1990 Jan;80(1):17–21. doi: 10.2105/ajph.80.1.17. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bobey D. G., Ederer G. M. Rapid detection of yeast enzymes by using 4-methylumbelliferyl substrates. J Clin Microbiol. 1981 Feb;13(2):393–394. doi: 10.1128/jcm.13.2.393-394.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bradbury J. M. Rapid biochemical tests for characterization of the Mycoplasmatales. J Clin Microbiol. 1977 May;5(5):531–534. doi: 10.1128/jcm.5.5.531-534.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burman L. G., Ostensson R. Time- and media-saving testing and identification of microorganisms by multipoint inoculation on undivided agar plates. J Clin Microbiol. 1978 Aug;8(2):219–227. doi: 10.1128/jcm.8.2.219-227.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chadwick P., Delisle G. J., Byer M. Biochemical identification of hospital enterobacteria by replica agar plating. Can J Microbiol. 1974 Dec;20(12):1653–1664. doi: 10.1139/m74-257. [DOI] [PubMed] [Google Scholar]
  7. Chomarat M., Lepape A., Riou J. Y., Flandrois J. P. Septicémie à Pseudomonas picketti. Pathol Biol (Paris) 1985 Jan;33(1):55–56. [PubMed] [Google Scholar]
  8. Fass R. J., Barnishan J. Letter: Acute meningitis due to a Pseudomonas-like Group Va-1 bacillus. Ann Intern Med. 1976 Jan;84(1):51–52. doi: 10.7326/0003-4819-84-1-51. [DOI] [PubMed] [Google Scholar]
  9. Fujita S., Yoshida T., Matsubara F. Pseudomonas pickettii bacteremia. J Clin Microbiol. 1981 Apr;13(4):781–782. doi: 10.1128/jcm.13.4.781-782.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gardner S., Shulman S. T. A nosocomial common source outbreak caused by Pseudomonas pickettii. Pediatr Infect Dis. 1984 Sep-Oct;3(5):420–422. doi: 10.1097/00006454-198409000-00006. [DOI] [PubMed] [Google Scholar]
  11. Graber C. D., Jervey L. P., Ostrander W. E., Salley L. H., Weaver R. E. Endocarditis due to a lanthanic, unclassified Gram-negative bacterium (group IV d). Am J Clin Pathol. 1968 Feb;49(2):220–223. doi: 10.1093/ajcp/49.2.220. [DOI] [PubMed] [Google Scholar]
  12. Grange J. M., Clark K. Use of umbelliferone derivatives in the study of enzyme activities of mycobacteria. J Clin Pathol. 1977 Feb;30(2):151–153. doi: 10.1136/jcp.30.2.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Haertl R., Bandlow G. Epidemiological fingerprinting of Enterobacter cloacae by small-fragment restriction endonuclease analysis and pulsed-field gel electrophoresis of genomic restriction fragments. J Clin Microbiol. 1993 Jan;31(1):128–133. doi: 10.1128/jcm.31.1.128-133.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kahan A., Philippon A., Paul G., Weber S., Richard C., Hazebroucq G., Degeorges M. Nosocomial infections by chlorhexidine solution contaminated with Pseudomonas pickettii (Biovar VA-I). J Infect. 1983 Nov;7(3):256–263. doi: 10.1016/s0163-4453(83)97196-7. [DOI] [PubMed] [Google Scholar]
  15. King A., Holmes B., Phillips I., Lapage S. P. A taxonomic study of clinical isolates of Pseudomonas pickettii, 'P. thomasii' and 'group IVd' bacteria. J Gen Microbiol. 1979 Sep;114(1):137–147. doi: 10.1099/00221287-114-1-137. [DOI] [PubMed] [Google Scholar]
  16. Kostman J. R., Edlind T. D., LiPuma J. J., Stull T. L. Molecular epidemiology of Pseudomonas cepacia determined by polymerase chain reaction ribotyping. J Clin Microbiol. 1992 Aug;30(8):2084–2087. doi: 10.1128/jcm.30.8.2084-2087.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kämpfer P., Rauhoff O., Dott W. Glycosidase profiles of members of the family Enterobacteriaceae. J Clin Microbiol. 1991 Dec;29(12):2877–2879. doi: 10.1128/jcm.29.12.2877-2879.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lacey S., Want S. V. Pseudomonas pickettii infections in a paediatric oncology unit. J Hosp Infect. 1991 Jan;17(1):45–51. doi: 10.1016/0195-6701(91)90076-k. [DOI] [PubMed] [Google Scholar]
  19. Maki D. G., Klein B. S., McCormick R. D., Alvarado C. J., Zilz M. A., Stolz S. M., Hassemer C. A., Gould J., Liegel A. R. Nosocomial Pseudomonas pickettii bacteremias traced to narcotic tampering. A case for selective drug screening of health care personnel. JAMA. 1991 Feb 27;265(8):981–986. [PubMed] [Google Scholar]
  20. McNeil M. M., Solomon S. L., Anderson R. L., Davis B. J., Spengler R. F., Reisberg B. E., Thornsberry C., Martone W. J. Nosocomial Pseudomonas pickettii colonization associated with a contaminated respiratory therapy solution in a special care nursery. J Clin Microbiol. 1985 Dec;22(6):903–907. doi: 10.1128/jcm.22.6.903-907.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Mierendorf R. C., Pfeffer D. Direct sequencing of denatured plasmid DNA. Methods Enzymol. 1987;152:556–562. doi: 10.1016/0076-6879(87)52061-4. [DOI] [PubMed] [Google Scholar]
  22. Miller J. M., Rhoden D. L. Preliminary evaluation of Biolog, a carbon source utilization method for bacterial identification. J Clin Microbiol. 1991 Jun;29(6):1143–1147. doi: 10.1128/jcm.29.6.1143-1147.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Minah G. E., Rednor J. L., Peterson D. E., Overholser C. D., Depaola L. G., Suzuki J. B. Oral succession of gram-negative bacilli in myelosuppressed cancer patients. J Clin Microbiol. 1986 Aug;24(2):210–213. doi: 10.1128/jcm.24.2.210-213.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Murray B. E., Singh K. V., Heath J. D., Sharma B. R., Weinstock G. M. Comparison of genomic DNAs of different enterococcal isolates using restriction endonucleases with infrequent recognition sites. J Clin Microbiol. 1990 Sep;28(9):2059–2063. doi: 10.1128/jcm.28.9.2059-2063.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. O'Brien M., Davis G. H. Enzymatic profile of Pseudomonas maltophilia. J Clin Microbiol. 1982 Sep;16(3):417–421. doi: 10.1128/jcm.16.3.417-421.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. O'Brien T. F., Ross D. G., Guzman M. A., Medeiros A. A., Hedges R. W., Botstein D. Dissemination of an antibiotic resistance plasmid in hospital patient flora. Antimicrob Agents Chemother. 1980 Apr;17(4):537–543. doi: 10.1128/aac.17.4.537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Osterhout G. J., Shull V. H., Dick J. D. Identification of clinical isolates of gram-negative nonfermentative bacteria by an automated cellular fatty acid identification system. J Clin Microbiol. 1991 Sep;29(9):1822–1830. doi: 10.1128/jcm.29.9.1822-1830.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Phillips I., Eykyn S., Laker M. Outbreak of hospital infection caused by contaminated autoclaved fluids. Lancet. 1972 Jun 10;1(7763):1258–1260. doi: 10.1016/s0140-6736(72)90981-6. [DOI] [PubMed] [Google Scholar]
  29. Roberts L. A., Collignon P. J., Cramp V. B., Alexander S., McFarlane A. E., Graham E., Fuller A., Sinickas V., Hellyar A. An Australia-wide epidemic of Pseudomonas pickettii bacteraemia due to contaminated "sterile" water for injection. Med J Aust. 1990 Jun 18;152(12):652–655. doi: 10.5694/j.1326-5377.1990.tb125422.x. [DOI] [PubMed] [Google Scholar]
  30. Trotter J. A., Kuhls T. L., Pickett D. A., Reyes de la Rocha S., Welch D. F. Pneumonia caused by a newly recognized pseudomonad in a child with chronic granulomatous disease. J Clin Microbiol. 1990 Jun;28(6):1120–1124. doi: 10.1128/jcm.28.6.1120-1124.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Verschraegen G., Claeys G., Meeus G., Delanghe M. Pseudomonas pickettii as a cause of pseudobacteremia. J Clin Microbiol. 1985 Feb;21(2):278–279. doi: 10.1128/jcm.21.2.278-279.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Wertheim W. A., Markovitz D. M. Osteomyelitis and intervertebral discitis caused by Pseudomonas pickettii. J Clin Microbiol. 1992 Sep;30(9):2506–2508. doi: 10.1128/jcm.30.9.2506-2508.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES