Table 5.

Real-time RT-PCR analysis of EXP5 and KCS1 gene expression.

	WT 40-dCT	exo 40-dCT	ddCT	FC
10 d
EXP5	38.4 ± 0.02	37.0 ± 0.02	1.4	2.6
KCS1	39.0 ± 0.02	37.9 ± 0.01	1.1	2.1
30 d
EXP5	37.2 ± 0.19	36.2 ± 0.01	1.1	2.1
KCS1	37.7 ± 0.43	36.4 ± 0.01	1.3	2.5
50 d
EXP5	35.1 ± 0.12	34.4 ± 0.17	0.7	1.6
KCS1	32.0 ± 0.12	31.5 ± 0.06	0.6	1.5

Wild-type and exo plants were grown in soil. RNA was extracted from rosette leaves of 10-, 30-, and 50 day-old plants. eIF1α C_T values were subtracted from respective C_T values of the gene of interest resulting in dC_T. Subsequently, differences were subtracted from an arbitrary value (i.e., 40). Higher numbers indicate higher transcript levels. ddC_T results from subtracting (40 – dC_T)_exo from (40 – dC_T)_WT. A ddC_T of one cycle indicates a fold change (FC, wild-type vs. exo) of two. Error: SE of gene of interest in three technical replicates. The experiment was carried out independently three times, representative examples of observed values are shown. For sequences of real-time RT-PCR primer pairs see [7].