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. 2008 Oct 23;117(3):379–386. doi: 10.1289/ehp.11865

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Direct interaction of TPT and the cellular proteasome. The 26S proteasome immunoprecipitates were prepared from MDA-MB-231 cells treated with 10 μmol/L TPT or methanol for 2 hr. The whole-cell extracts were immunoprecipitated with agarose beads conjugated with 20S proteasome subunit α2 antibody. Abbreviations: Sol, solvent; Supern, supernatant. (A) Associated proteasomal CT-like activity; (B) Proteasomal β5 protein level; (C ) Total Sn level measured by ICP-MS. Aprotinin immunocomplexes prepared from the cells treated with TPT were used as a nonspecific TPT binding control. Data are mean ±SD of three experiments.

**p < 0.01.