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. 2009 Mar 31;7(3):e1000073. doi: 10.1371/journal.pbio.1000073

Figure 3. Interaction of p65 with Mediator Drives Recruitment of pol-II.

Figure 3

(A) Trap-80 knock-down does not prevent p65 recruitment to its target promoters.

(B) Trap-80 knock-down blocks pol-II recruitment to Trap-80 dependent promoters.

(C) Trap-80 knock-down blocks TFIIB recruitment to Trap-80 dependent promoters.

(D) Presence of Trap-80 at promoter regions.

(E) Trap-80 knock-down blocks Med-26 recruitment to Trap-80 dependent promoters.

ChIP using antibodies against p65 (A), pol-II (B), TFIIB (C), Trap-80 (D), and Med-26 (E), after stimulation with TNF-α. Bound DNA was analyzed using primers and probes specific for promoter regions. The amounts are presented as the percentage recovered out of the total input DNA (percent input). All measurements were performed in duplicate; the results presented here are representative of two to five experiments.