Figure 6. p65 Is Required for Promoter Occupancy by Secondary Transcription Factors.
(A) Positions of promoter-proximal NF-κB binding motifs relative to the transcriptional start site. Cumulative percentage of promoters in which the closest conserved NF-κB binding motif lies within the indicated distance to the TSS. Promoters analysed are those of the top 30 Trap-80–dependent and 30 Trap-80–independent genes, from amongst the 200 most induced by TNF-α.
(B) Recruitment of p65 and secondary transcription factors to the Mip-2, Nfkbia, and Ip-10 promoters in wild-type and p65-knockout fibroblasts. ChIP using antibodies against p65, Sp1, c-Jun, JunD, or ATF-3, after stimulation with TNF-α. The results presented here are representative of two to three experiments.