FIGURE 1.
Purification of recombinant PMI1 and PMI2 proteins. Recombinant PMI1 and PMI2 proteins were expressed in E. coli, purified with Ni2+ affinity chromatography, and verified using SDS-PAGE with Coomassie Blue staining. The experimental conditions are described under”Experimental Procedures.“The left lane and right lane of each PMI contain 15 μg of crude extract and 2 μg of purified recombinant protein, respectively. M, molecular mass standards (Amersham Biosciences) as indicated on the left. E, empty pCold II vector.