FIGURE 4.

Expressions and total activity levels of Arabidopsis PMI isoenzymes and AsA concentrations in different organs of A. thaliana. Various organs, including the leaf (L), stem (S), root (R), cauline leaf (CL), and inflorescence (IN) of 5-week-old Arabidopsis plants were harvested at 4 h after the illumination. A, relative levels of PMI1 and PMI2 were determined by real time PCR. Relative levels of the transcripts were normalized to Actin2 mRNA (= 1%). Error bars indicate S.D. (n = 3). B, Western blot of PMI1 protein. C, total PMI activity in crude extracts of each organ was measured by the coupling reaction as described under”Experimental Procedures.“Error bars indicate S.D. (n = 3). D, AsA and DHA concentrations in each organ. Error bars indicate S.D. (n = 3).