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Homoaconitase and homoisocitrate dehydrogenase specifically converted (R)-homocitrate to 2-oxoadipate in a coupled reaction. The reaction progress curves show the NADH absorbance in reactions containing 0.5 mm (R)-homocitrate (solid circles), 0.5 mm (S)-homocitrate (open squares), or no substrate (solid squares). A control reaction without homoaconitase enzyme also showed no NADH production (data not shown).
