FIGURE 5.
SCF-stimulated PI3K signaling is not affected in PTPα-/- BMMCs. Wild-type (PTPα+/+) and PTPα-/- BMMCs were left untreated (0) or stimulated with SCF for 5, 15, and 30 min as indicated. A, c-Kit immunoprecipitates were probed for phosphotyrosine (P-Tyr), p85, and c-Kit. The blot shown is representative of three experiments, but no change in p85 binding to c-Kit was observed in two other experiments (not shown). B, cell lysates were probed for phospho-Ser473-Akt and Akt. Quantified results from three such experiments are shown in the graph. The bars represent the mean ± S.D. and the asterisks depict a significant difference (p < 0.01) between PTPα+/+ (gray bars) and PTPα-/- (black bars) BMMCs.