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. 2008 Oct 24;283(43):29175–29185. doi: 10.1074/jbc.M804077200

FIGURE 7.

FIGURE 7.

Reduced Rac and Cdc42 activation by SCF in PTPα-/- BMMCs. Wild-type (PTPα+/+) and PTPα-/- BMMCs were left untreated (0) or stimulated with SCF for 5, 15, and 30 min. Lysates were used for GST-PBD binding assays, and the GST-PBD complexes (upper panels) and cell lysates (lower panels) were analyzed by immunoblotting for Rac2 (A), Rac1 (B), and Cdc42 (C). Independent pulldown assays were used to detect activated Rac2, Rac1, and Cdc42. D, cell lysates were probed for phospho-PAK and PAK. E, Vav1 immunoprecipitates were probed for phosphotyrosine (P-Tyr) and Vav1. The results of several experiments (Rac2, Rac1, Cdc42, and PAK, n = 3; Vav1, n = 4) were quantified by densitometry, and are shown as mean ± S.D. in the bar graphs. Asterisks indicate a significant difference (p < 0.05) between wild-type (gray bars) and PTPα-/- (black bars) cells.