FIGURE 1.

Identification of interacting partners for FLAG-PP4c. A, HEK293 cells were stably transfected with pcDNA₃-FLAG-PP4c or pcDNA₃-FLAG alone. After G418 selection, equal amounts of lysate were separated by SDS-PAGE, and immunoblots (IB) were performed using an anti-PP4c antibody. Arrows indicate the positions of endogenous and epitope-tagged PP4c. B, Venn diagram showing the overlap between the interactors identified from TAP-PP4c purifications (gray; as per Gingras et al. (18)) and the FLAG-PP4c purifications (see Table 1 for details).