FIGURE 5.

DMOG and iron chelators, such as 2,2′-dipyridyl and deferoxamina, are unable to up-regulate the HIF-1α double proline mutant. A and B, stable cells expressing mHIF-1α (wt 4), or mHIF-1α(P402A/P563A) (PP-A 9) were treated with DMOG, 2,2′-dipyridyl (Dp), or deferoxamine (DFX) for the indicated times, or kept at normoxia (N) or treated with CoCl₂ (Co) for constant end-point experiments. Whole cell extracts were separated by SDS-PAGE and analyzed by immunoblotting using anti-FLAG (α-FLAG), or anti-actin (α-Actin) antibodies.