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. 2008 Oct 24;283(43):29385–29395. doi: 10.1074/jbc.M803089200

FIGURE 2.

FIGURE 2.

Internal dynamics and chemical shift perturbations upon deprotonation of Glu547 carboxyl group of EphA1tm. A, local effective rotation correlation times (τR) for the EphA1tm dimer embedded in the DMPC/DHPC bicelles at pH 4.3 (dashed line) and pH 6.3 (solid line). Uncertainties are shown by bars. B, the HN chemical shift differences for the major form of the EphA1tm dimer embedded in the DMPC/DHPC bicelles at pH 6.3 and pH 4.3. The chemical shift uncertainty is shown by a bar in the left top corner. In the inset panel the pH dependence of HN chemical shift of Thr544 is presented. The experimental and theoretical sigmoid-like curves with Hill coefficients equal to 1.5 and 1 are shown by dashed and solid black lines, respectively. C, the HN chemical shift differences between major and minor components of doubling cross-peaks in the 1H-15N HSQC spectra acquired for the EphA1tm dimer embedded in the DMPC/DHPC bicelles at pH 6.3. Areas of the Gly558 and Ala559 residues subjected to strong HN resonance broadening in the NMR spectra over the entire pH range used are shaded.