FIGURE 1.

A, time course of the chlorophyll fluorescence relative yield of LHCII incorporated in polyacrylamide gel. Fluorescence was excited at 650 nm and measured in the 685-800 nm region. The arrow labeled -DM indicates an LHCII gel placed in detergent-free medium. The red trace indicates incubation of the gel containing LHCII pretreated with glutaraldehyde for 5 min before setting the gel. After 60 min, recovery of the fluorescence of the samples was achieved by placing the gel fragment in medium containing 0.03% n-dodecyl β-d-maltoside (arrow labeled +DM); the control sample (green circles) was incubated in this buffer throughout the experiment. The blue circles indicate an LHCII gel placed in recovery buffer additionally containing glutaraldehyde. rel., relative. B, time-correlated single-photon counting fluorescence measurements of unquenched (upper panel, blue trace) and quenched (red trace) LHCII in the gel. The lower panels display the difference between the three-exponential fit and the experimental data.