FIGURE 4.

Diffusional mobilities of raft- and non-raft-associated proteins at the level of the Golgi compartment in polarized MDCK cells. A, apparent diffusion coefficients of our studied proteins at steady state (colored bars) and upon depletion of cholesterol by inhibition of HMG-CoA reductase (white bars). B, apparent diffusion coefficients of our studied proteins at steady state (colored bars) and upon loading of cholesterol (white bars). These experiments have been performed at least three independent times, n > 15. The error bars are the means ± S.D. with ^*, p < 0.0001. C, analysis by velocity gradient of oligomerization status of basolaterally sorted proteins (GFP-PrP and GFP-PIT) at steady state or upon loading of cholesterol.