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. 2008 Oct 31;283(44):29671–29680. doi: 10.1074/jbc.M802785200

FIGURE 1.

FIGURE 1.

E2 induces BRCA2 expression in MCF-7 cells. A and B, E2 induces BRCA2 mRNA and protein levels in MCF-7 cells. MCF-7 cells were precultured for 4 days in phenol red-free RPMI 1640 medium containing 5% charcoal dextran-stripped FBS, and then MCF-7 cells were cultured for 24 h in RPMI 1640 medium plus 10 nm E2. A, mRNA expression levels of BRCA2. B, protein expression levels of BRCA2. C, E2 induces BRCA2 promoter activity in transiently transfected MCF-7 cells. MCF-7 cells were precultured for 4 days in phenol red-free RPMI 1640 medium containing 5% charcoal dextran-stripped FBS, and then BRCA2 promoter construct (pGL3-BRCA2) was transiently transfected into MCF-7 cells, and cells were cultured for 24, 48, or 72 h in RPMI 1640 medium or RPMI 1640 medium plus 10 nm E2. Luciferase activity in the cells treated with E2 was compared with that in the control cells. **, p < 0.01. D, E2 induces BRCA2 promoter activity with the help of ERα in transiently transfected MDA-MB-231 cells. MDA-MB-231 cancer cells were co-transfected with pGL3-BRCA2 with ERα expression vector or an empty vector (pcDNA3). Transfected cells were cultured for 24 h in RPMI 1640 medium or RPMI 1640 medium plus 10 nm E2. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.