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. 2008 Oct 31;283(44):29671–29680. doi: 10.1074/jbc.M802785200

FIGURE 5.

FIGURE 5.

Detection of ERα, ERβ, MyoD, p53, or CtBP1 transcriptional complex, respectively, by co-IP assays. The process was described under “Experimental Procedures.” A, cell lysates extracted from ERα-overexpressing MCF-7 cells were subjected to immunoprecipitation with IgG or ERα antibody followed by immunoblotting with ERα, CBP, p300, MyoD, Sp1, or ERβ antibody, respectively. B, cell lysates extracted from ERβ-overexpressing MCF-7 cells were subjected to immunoprecipitation with IgG or ERβ antibody followed by immunoblotting with ERα, CBP, p300, MyoD, Sp1, or ERβ antibody, respectively. C, cell lysates extracted from MyoD-overexpressing MCF-7 cells were subjected to immunoprecipitation with IgG or MyoD antibody followed by immunoblotting with MyoD, p68, p72, ERα, or ERβ antibody, respectively. D, cell lysates extracted from p53-overexpressing MCF-7 cells were subjected to immunoprecipitation with IgG or p53 antibody followed by immunoblotting with HDAC1, CtBP1, Sp1, or p53 antibody, respectively. E, cell lysates extracted from CtBP1-overexpressing MCF-7 cells were subjected to immunoprecipitation with IgG or CtBP1 antibody followed by immunoblotting with HDAC1, p53, CtBP1, and CtBP2 (Santa Cruz Biotechnology, Inc.) antibodies, respectively.