FIGURE 3.

Effects of XP620 on the in vitro acylation of 1,2-DOG and 2-MOG by Caco-2 and rat intestinal membrane extracts. Membrane fractions derived from Caco-2 cells differentiated for 2 weeks (A–C) or rat intestinal mucosal membranes (D–F) were used for enzymatic assays for acylation of 1,2-DOG (A and D) and 2-MOG (B, C, E, and F). Aliquots of 30 μg of Caco-2 cells or 5 μg of rat intestinal mucosal membrane proteins were incubated with 50 μm 1,2-DOG or 2-MOG and 50 μm [¹⁴C]oleoyl-CoA for 10 min in Assay Buffer 2 in the absence or presence of the indicated concentrations of XP620. The reactions were stopped by chloroform/methanol (2:1, v/v), and the organic extracts were subjected to TLC analysis as described under “Experimental Procedures.” The specific activities of ¹⁴C incorporated into the DAG and TAG bands were calculated as nmol/min/mg. Results shown are representative of three independent experiments with similar results. All points are means of duplicate determinations.