DNA-PK is dispensable for PKB Ser-473 phosphorylation upon insulin and
growth factor stimulation. After overnight fasting and insulin
(ins) stimulation (1 unit/kg body weight) for 20 min, tissues from
3-month-old (WT) and DNA-PKcs-/- (KO) mice were
analyzed for PKB Ser-473 phosphorylation via immunoblotting with
phospho-specific antibodies. A, skeletal muscle; B, liver;
C, heart; D, adipose tissue. Wild-type and
DNA-PKcs-/- MEFs (KO) were stimulated with IGF-1 (50 ng/ml)
(E) or serum (10%) (F) for various times, and PKB Ser-473
phosphorylation was analyzed by immunoblotting.