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. 2008 Oct 31;283(44):30025–30033. doi: 10.1074/jbc.M803053200

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Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Deletion of PKBα prevents neoplastic expansion of DNA-PKcs^-/- thymocytes. A, histological analysis of thymi from wild-type (WT), DNA-PKcs^-/- (KO), and DNA-PKcs^-/-PKBα^-/- double knock-out (DKO) mice and thymic tumors from DNA-PKcs^-/- mice. Thymi were fixed with 4% paraformaldehyde, processed with paraffin, sectioned, and stained with hematoxylin-eosin (×400 magnification; scale bar indicates 50 μm). The insets show ×40 magnification. M, medulla; C, cortex. B, flow cytometric analysis of thymocytes from WT, DNA-PKcs^-/- (KO), and DNA-PKcs^-/-PKBα^-/- (DKO) mice for CD4 and CD8 markers.