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Effects of AML1b on the activity of c-mpl promoter. A, 293T cells were transfected with 2 μg of AML1b, PEBP2β, and 2 μg of indicated reporter gene. The relative firefly luciferase (LUC) activities were calculated by normalizing transfection efficiencies according to the Renilla luciferase activities. The results are shown as the means ± S.D. of triplicate cultures. B, HeLa cells were transfected with 0.5 μg of AML1b and PEBP2β and 1 μg of reporter genes by FuGENE 6.
