Skip to main content
. 2008 Oct 31;283(44):30045–30056. doi: 10.1074/jbc.M804768200

FIGURE 5.

FIGURE 5.

Effects of AML1dC on c-Mpl expression on hematopoietic progenitor cells and megakaryocytes and megakaryopoiesis. A, experimental design using the OP9 system. ES cells were deprived of leukemia inhibitory factor and cultured on OP9 cells for 4.5 days. Then Flk-1+ cell were sorted, replated onto OP9 cells, and cultured with mSCF and hTPO (for the analysis of c-Mpl expression in hematopoietic stem/progenitor cells) or only hTPO (for the analysis of megakaryocytic differentiation) with or without Tet for the time indicated. B, c-Mpl expression of nonadherent cells was examined by the direct immunofluorescence method on day 8.5 and day 12.5. The percentage of each fraction is indicated. The relative frequency of GFP- fraction in cultured cells with Tet and the relative frequency of GFP+ fraction in cultured cells without Tet were shown in parentheses. C, c-Mpl expression on early hematopoietic cells that derived from WT and AML1+/- ES cells on day 8.5. D and E, after 12.5-day cultures with TPO, megakaryocytic cells, which derived from ES cells expressing AMLdC, were subjected to morphological analysis (D), and DNA content analysis by propidium iodide staining (E).