FIGURE 6.

AML1 forms different transcriptional complexes on the c-mpl promoter in hematopoietic stem/progenitor cells and megakaryocytes. A, experimental design of ChIP-ReChIP assays. Murine Lin^-Sca1⁺ cells were cultured for 3 days with mSCF (50 ng/ml), mIL-3 (10 ng/ml), and hTPO (50 ng/ml). These cultured cells and CMK cells were cross-linked and subjected to the ChIP-ReChIP assays. B, after the second immunoprecipitation (IP), PCR analyses were performed using the primer set shown in Fig. 2 with the eluted DNA as a template.