FIGURE 2.

Human S100A6 promoter-luciferase reporter constructs. A, using the full-length pBS construct, shorter fragments of the human S100A6 gene (solid boxes were prepared by restriction digests to generate deletion constructs). Restriction enzymes are abbreviated as follows: B, BamHI; Sc, ScaI; N, NcoI; Sp, SphI; X, XhoI; Sm, SmaI. The black arrows (luciferase (Luc)) represent the coding region of firefly luciferase. B, neonatal rat cardiac myocytes, cardiac fibroblasts, and aortic smooth muscle cells were co-transfected with the appropriate S100A6 promoter-luciferase construct and RSV-CAT. The bars shown are the mean ± S.E. of luciferase expression (normalized to the RSV-CAT activity) relative to pGBS-167 in the same cell type, which has been assigned the value of 1. ^*, p < 0.05 relative to pGBS-167. n = 6.